M8280
Murashige and Skoog Modified Basal Salt Mixture
powder, suitable for plant cell culture
form
powder
nitrogen analysis
40 mM total
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
Application
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Biochem/physiol Actions
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M8280) contain the macronutrients and micronutrients of the original classic formulation with sodium nitrate substituted for ammonium nitrate. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
Preparation Note
Modified by replacing ammonium nitrate with sodium nitrate.
Other Notes
NH4-free
With the macro- and micronutrients as described by Murashige and Skoog (1962).
With the macro- and micronutrients as described by Murashige and Skoog (1962).
Regulatory Information
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Ca2+ regulates reactive oxygen species production and pH during mechanosensing in Arabidopsis roots.
Gabriele B Monshausen et al.
The Plant cell, 21(8), 2341-2356 (2009-08-06)
Mechanical stimulation of plants triggers a cytoplasmic Ca(2+) increase that is thought to link the touch stimulus to appropriate growth responses. We found that in roots of Arabidopsis thaliana, external and endogenously generated mechanical forces consistently trigger rapid and transient
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