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M9194

Sigma-Aldrich

MDR1 human

membrane preparation, for ATPase, recombinant, expressed in Sf9 cells

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Synonym(s):
ABCB1, Pgp
MDL number:
MFCD08705568

biological source

human

recombinant

expressed in Sf9 cells

form

membrane preparation

technique(s)

drug transporter assay: suitable

UniProt accession no.

P08183

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... ABCB1(5243)

Diagnostic Implications

The MDR1 protein is involved in cancer drug resistance and in the transport of hydrophobic drugs and xenobiotics in the bowel, kidney, liver, and the blood-brain barrier. Drugs interacting with this protein may be useful for the reversal of cancer drug resistance or increasing the absorption or brain entry of various pharmacological agents.

Specifications

Detection of ATPase activity of the MDR1 protein is a measure of transporter activity. The assay is performed using purified membrane vesicles from Sf9 (Spodoptera frugiperda) cells, expressing high levels of MDR1 protein. The ABC transporters pump substrates out of the cell by using hydrolysis of ATP as an energy source. ATP hydrolysis yields inorganic phosphate (Pi), which can be detected by a simple colorimetric reaction. The amount of Pi liberated is proportional to the activity of the transporter.

Physical form

Supplied as isolated Sf9 cell membranes containing human MDR1 (Pgp) suspended in TMEP solution.

Analysis Note

Membrane preparations containing ABC transporters show a baseline ATPase activity that varies for different transporters. Transported substrates increase this baseline ATPase activity, while inhibitors or slowly transported compounds inhibit the baseline ATPase activity and/or the ATPase activity measured in the presence of a stimulating agent. Both activation and inhibition studies can be performed.

Legal Information

Distributed for SOLVO Biotechnology, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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