M9194
MDR1 human
membrane preparation, for ATPase, recombinant, expressed in Sf9 cells
Synonym(s):
ABCB1, Pgp
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About This Item
UNSPSC Code:
12161501
MDL number:
biological source
human
recombinant
expressed in Sf9 cells
form
membrane preparation
technique(s)
drug transporter assay: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... ABCB1(5243)
General description
The MDR1 protein is involved in cancer drug resistance and in the transport of hydrophobic drugs and xenobiotics in the bowel, kidney, liver, and the blood-brain barrier. Drugs interacting with this protein may be useful for the reversal of cancer drug resistance or increasing the absorption or brain entry of various pharmacological agents.
Features and Benefits
Detection of ATPase activity of the MDR1 protein is a measure of transporter activity. The assay is performed using purified membrane vesicles from Sf9 (Spodoptera frugiperda) cells, expressing high levels of MDR1 protein. The ABC transporters pump substrates out of the cell by using hydrolysis of ATP as an energy source. ATP hydrolysis yields inorganic phosphate (Pi), which can be detected by a simple colorimetric reaction. The amount of Pi liberated is proportional to the activity of the transporter.
Physical form
Supplied as isolated Sf9 cell membranes containing human MDR1 (Pgp) suspended in TMEP solution.
Analysis Note
Membrane preparations containing ABC transporters show a baseline ATPase activity that varies for different transporters. Transported substrates increase this baseline ATPase activity, while inhibitors or slowly transported compounds inhibit the baseline ATPase activity and/or the ATPase activity measured in the presence of a stimulating agent. Both activation and inhibition studies can be performed.
Legal Information
Distributed for SOLVO Biotechnology, Inc.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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