biological source
rat
Quality Level
recombinant
expressed in E. coli
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
specific activity
500 U/mg (Can be activated by MEK to more than 500 units per mg)
mol wt
42 kDa
UniProt accession no.
application(s)
cell analysis
storage temp.
−20°C
Gene Information
rat ... Mapk1(116590)
Biochem/physiol Actions
MAP kinase is a serine/threonine kinase that is activated by MEK through a phosphorylation cascade. The cascade starts with extracellular signals transmitted by growth factor-regulated receptor tyrosine kinases or G-protein coupled receptors. Dually phosphorylated (Thr and Tyr) MAP kinase translocates from the cytoplasm to the nucleus, where it phosphorylates protein transcription factors, transducing extracellular signals into genomic responses, proliferation or differentiation.
Unit Definition
One unit of activated MAP kinase will transfer 1 nmol of phosphate from ATP to myelin basic protein per min at 30 °C.
Physical form
Lyophilized powder containing Tris, pH 7.5, NaCl, and EGTA, with DTT and trehalose as stabilizers
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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