M9532
甘露醇脱氢酶 来源于肠系膜明串珠菌
lyophilized powder, ≥60 units/mg protein
别名:
甘露醇:NAD 氧化还原酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥60 units/mg protein
form
lyophilized powder
Quality Segment
specific activity
≥60 units/mg protein
mol wt
136 kDa
storage temp.
−20°C
General description
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.
The molecular weight of mannitol dehydrogenase is 136 kDa.
Application
Can be used for the production of D-mannitol.
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
This preparation is useful in the determination of mannitol in urine.
Biochem/physiol Actions
Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of D-mannitol to D-fructose. Mannitol dehydrogenase (MDH) obtained from L. mesenteroides is highly specific for the conversion fructose and mannitol.
Physical form
含缓冲盐、磷酸钾以及二硫苏糖醇的冻干粉
Preparation Note
部分纯化
Analysis Note
根据 Yamanaka, Meth. Enzymol., 41(B), 138 (1975) 所述方法进行分析。
Other Notes
在 pH 5.3 和 30°C 且存在 NADH 的条件下，一单位本品每分钟将还原 1.0μmol D-果糖。如上定义的一单位酶相当于如 Lunn 等人所述的约 1.8 单位酶，并且在 pH 8.6 和 40°C 且存在 NAD 的条件下，一单位酶每分钟将氧化 1.0μmol D-甘露醇。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves