M9817
Anti-MBD4 antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, WB
Citations:
9
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
mouse
concentration
~1 mg/mL
technique(s)
microarray: suitable, western blot: 0.5-1 μg/mL using nuclear extracts of K-562 cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MBD4(8930)
mouse ... Mbd4(17193)
General description
Human MBD4 protein encodes an alternative spliced form which lacks C-terminal 42 amino acids.
Methyl-CpG binding domain protein 4 (MBD4) is a thymine glycosylase which possesses a carboxy-terminal DNA glycosylase domain and an amino-terminal MBD domain. It belongs to the methyl-CpG-binding protein family.
Immunogen
synthetic peptide corresponding to amino acids 541-554 of mouse MBD4 conjugated to KLH via an N-terminal added cysteine residue. The sequence is conserved in human.
Application
Anti-MBD4 antibody produced in rabbit has been used in immunoblotting and chromatin-immunoprecipitation (ChIP).
Biochem/physiol Actions
Methyl-CpG binding domain protein 4 (MBD4) plays an important role in DNA demethylation. It can remove mismatched bases which are present with a guanine. The preferred substrate for MBD4 is thymine over N4-ethenocytosine.
The methyl-CpG binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches, the primary product of deamination at methyl-CpG. The combined specificities of binding and catalysis indicates that this protein may function to minimize mutation at methylCpG. in vivo, the DNA repair properties of MBD4 suggests that it plays a role in suppressing mutability and tumorigenesis.
Physical form
Solution in 0.01 M phosphate buffered saline containing 1% bovine serum albumin (BSA) and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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The thymine glycosylase MBD4 can bind to the product of deamination at methylated CpG sites
Hendrich B, et al.
Nature, 401(6750), 301-301 (1999)
MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress
Laget S, et al.
Epigenetics, 9(4), 546-556 (2014)
Identification and characterization of a family of mammalian methyl CpG-binding proteins
Hendrich B and Bird A
Genetics research, 72(1), 59-72 (1998)
Enhanced CpG mutability and tumorigenesis in MBD4-deficient mice
Millar CB, et al.
Science, 297(5580), 403-405 (2002)
Hideharu Hashimoto et al.
Nucleic acids research, 40(17), 8276-8284 (2012-06-29)
The mammalian DNA glycosylase--methyl-CpG binding domain protein 4 (MBD4)--is involved in active DNA demethylation via the base excision repair pathway. MBD4 contains an N-terminal MBD and a C-terminal DNA glycosylase domain. MBD4 can excise the mismatched base paired with a
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