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MAK008

Sigma-Aldrich

Hydroxyproline Assay Kit

sufficient for 100 colorimetric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

dermatological diseases

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Hydroxyproline (4-hydroxyproline) is a non-proteinogenic amino acid formed by the post-translational hydroxylation of proline. Hydroxyproline is a major component of collagen, where it serves to stabilize the helical structure. Because hydroxyproline is largely restricted to collagen, the measurement of hydroxylproline levels can be used as an indicator of collagen content. Conditions that increase collagen turnover can elevate serum and urine hydroxyproline levels. Urine and serum hydroxyproline levels can be used as a marker for bone resorption.

Application

Hydroxyproline Assay Kit has been used to determine the concentration of hydroxyproline and collagen.

Suitability

Suitable for hydroxyproline detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

In the Hydroxyproline Assay Kit, hydroxyproline concentration is determined by the reaction of oxidized hydroxyproline with 4-(Dimethylamino)benzaldehyde (DMAB), which results in a colorimetric (560 nm) product, proportional to the hydroxyproline present.

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Ox. Liq. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system, Thyroid

Supplementary Hazards

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

64.4 °F

Flash Point(C)

18 °C

Regulatory Information

危险化学品
易制爆化学品

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can Product MAK008, Hydroxyproline Assay Kit, be used to measure the hydroxyproline levels in cell culture supernatants?

    Yes.The manufacturer of the kit recommends that cell culture media (that is, cell culture supernatants) should be treated the same as serum or urine samples.

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