MAK021
High Sensitivity Glucose-6-Phosphate Assay Kit
sufficient for 100 fluorometric tests
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer; hematological disorder
storage temp.
−20°C
General description
Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high. G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. The Glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples.
Application
Suitable for quantifying Glucose-6-Phosphate concentrations in a variety of samples.
Biochem/physiol Actions
Glucose-6-phosphate concentration is by a enzyme assay, which results in a fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the G6P present. Typical sensitivities of detection for this kit are between 100-500 pmoles. The Glucose-6-Phosphate Assay Kit is much more sensitive than the Glucose-6-Phosphate Colorimetric Assay Kit (MAK014) and is suitable for samples with low concentrations of glucose-6-phosphate.
Features and Benefits
Compatible with high-throughput handling systems.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Regulatory Information
监管及禁止进口产品
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Role of the liver in the control
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of carbohydrate and lipid homeostasis
Postic C, et al.
Diabete & Metabolisme, 30, 398-408 (2004)
Glucose 6-phosphate dehydrogenase deficiency: from genotype to phenotype.
Lucio L.
The Hematology Journal : The Official Journal of the European Haematology Association / EHA, 1303-1306 (2006)
Glucose-6-phosphate dehydrogenase, NADPH, and cell survival.
Robert C S.
IUBMB Life, 64(5), 362-369 (2012)
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本页面介绍了一篇有关瓦博格效应的文章，以及其如何能够在正常氧气水平下，增强肿瘤细胞中葡萄糖向乳酸的转化。Otto Heinrich Warburg在1924年证明，癌细胞显示出对糖酵解的依赖性增加，以满足他们的能量需求，无论是否有充足的氧气存在。
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