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MAK022

Sigma-Aldrich

Calcium Colorimetric Assay

sufficient for 250 colorimetric tests

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NACRES:
NA.84

usage

sufficient for 250 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

neurological disorders; hematological disorder

storage temp.

2-8°C

General description

Calcium, the most abundant mineral in the human body, is a crucial intracellular element that is responsible for regulating many physiological and pathological processes. Calcium is found in either the free ion form or in bound complexes, for example the calcium phosphate and calcium carbonate complexes that make up bone tissue. Numerous physiological processes, including muscle contraction, cell adhesion, hormones/ neurotransmitters release, glycogen metabolism, cell proliferation/differentiation, blood clotting, nerve or synapthetic impulse transmission, and structural support of the skeleton are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems may be associated with certain human diseases.

Application

Calcium Colorimetric Assay kit has been used to measure calcium concentration in hippocampal samples and MC3T3-E1 mouse osteoblast cell line, which were cultured in osteogenic induction medium.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.

Principle

In this assay, the calcium ion concentration is determined by the chromogenic complex formed between calcium ions and o-cresolphthalein, which is measured at 575 nm and is proportional to the concentration of calcium ions present. The linear range of detection for this kit is between 0.4-2.0 μg.

replaced by

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H315 - H318 - H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Vitamin D, calcium homeostasis and aging
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