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MAK049

Sigma-Aldrich

Phosphatidylcholine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

neurological disorders; gastrointestinal diseases; cancer

storage temp.

−20°C

Related Categories

General description

Phosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.

Application

Phosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples.

Suitability

Suitable for the measurement of phosphatidylcholine in a variety of biological samples

Principle

In this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PC present.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Regulatory Information

常规特殊物品

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Copper-64 labeled liposomes for imaging bone marrow.
Sang-gyu L, et al.
Nuclear Medicine and Biology, 43(12), 781-787 (2016)
Riboflavin attenuates myocardial injury via LSD1-mediated crosstalk between phospholipid metabolism and histone methylation in mice with experimental myocardial infarction.
Peng W, et al.
Journal of Molecular and Cellular Cardiology, 115, 115-129 (2018)
Michelle S Prew et al.
Nature communications, 13(1), 3669-3669 (2022-06-28)
Very long-chain acyl-CoA dehydrogenase (VLCAD) is an inner mitochondrial membrane enzyme that catalyzes the first and rate-limiting step of long-chain fatty acid oxidation. Point mutations in human VLCAD can produce an inborn error of metabolism called VLCAD deficiency that can
Iron depletion induces hepatic secretion of biliary lipids and glutathione in rats
Alena P, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(12), 1469-1480 (2017)
Sang-Gyu Lee et al.
Nuclear medicine and biology, 43(12), 781-787 (2016-10-04)
Bone marrow is the soft tissue compartment inside the bones made up of hematopoietic cells, adipocytes, stromal cells, phagocytic cells, stem cells, and sinusoids. While [18F]-FLT has been utilized to image proliferative marrow, to date, there are no reports of
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