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Merck
CN

MAK056

Choline/Acetylcholine Quantification Kit

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

Synonym(s):

Choline/Acetylcholine Assay Kit

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161503

Key Documents

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Product Name

Choline/Acetylcholine Quantification Kit, sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

usage

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

detection method

colorimetric
fluorometric

storage temp.

−20°C

Application

Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.
Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Biochem/physiol Actions

In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.

General description

Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
8H25K06150
7L19K06150
8F16K06150
8H2506150
7D02K06150

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PloS one, 15(4), e0230472-e0230472 (2020-04-22)
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Phosphatidylcholine (PC), the most abundant of the phospholipids, has several metabolic functions in organs such as the liver and the intestine, important structural- and signaling functions in biological membranes, and might have a role in the effects of Roux-en-Y gastric
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Bek S, et al.
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Reactive oxygen species (ROS) are a common hallmark of many degenerative diseases, developing in all those cases where a failure of physiological antioxidant mechanisms occurs (in particular, antioxidant enzymes and the glutathione system), or in case of exposure to an
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Eliades L, et al.
Journal of Undergraduate Research, 1-6 (2014)
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