MAK057
Citrate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Citrate Test Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric, fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
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General description
Citrate is a key Tricarboxylic Acid (TCA) cycle intermediate formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA. Citrate is transported out of the mitochondria via the citrate-malate shuttle and converted back to acetyl-CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis via its actions on acetyl-CoA carboxylase and of glycolysis via its actions on phospho- fructokinase. Citrate metabolism and disposition can vary widely due to sex, age, and a variety of other factors including disease states. Cellular citrate levels are decreased in prostrate cancer cells and citrate levels may be a marker of prostrate cancer growth rate. The Citrate Assay Kit provides a simple, sensitive, and rapid means of quantifying citrate in a variety of samples.
Application
Suitable for the measurement of citrate in a variety of samples including tissue and cells
Citrate Assay kit has been used to determine the concentration of citrate in samples.
Biochem/physiol Actions
Citrate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the citrate present. Typical sensitivites of the Citrate Assay Kit are between 0.1 to 10 nmoles (2 μM-10 mM) of citrate in a variety of samples.
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
Regulatory Information
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