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MAK064

Lactate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Lactic Acid Assay Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 100 colorimetric or fluorometric tests

species reactivity

mouse, human

storage condition

dry at room temperature

technique(s)

activity assay: suitable

input

blood
cell

accession no.

P00338, NC_000011.10, NC_000073.7

application(s)

cosmetics
food and beverages

detection method

colorimetric, fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

Gene Information

human ... LDHA(3939)
mouse ... Ldha(16828)

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General description

L(+)-Lactate is a metabolic compound formed in animals by the action of the enzyme Lactate Dehydrogenase. Lactate is produced in proliferating cells and during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. This kit provides a convenient means for detecting L(+)-Lactate in biological samples. There is no need of pretreatment or purification of samples.

Application

Lactate Assay Kit has been used to determine the concentrations of lactate in samples.
Suitable for detecting L(+)-Lactate in culture medium, fermentation medium, blood and cells.

Biochem/physiol Actions

In this assay, lactate concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactate present. Typical levels of sensitivity for this kit are between 0.001-10 mM.

hcodes

H334

pictograms

Health hazard
GHS08

signalword

Danger

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 2

Hazard Classifications

Resp. Sens. 1

Regulatory Information

动植物源性产品

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Certificates of Analysis (COA)

Lot/Batch Number
8J15K06070
8A17K06070
8B23K06070
8L03K06070
8F30K06070

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Min Xu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 151-160 (2018-05-16)
Poor response to chemotherapy leads to the relapse and metastatic progression of tumors. Reprogrammed glucose metabolism is one of the important hallmarks of cancer that facilitates cancer cell survival, proliferation and chemoresistance. However, the precise fate of glucose metabolism and
Cardiac STAT3 Deficiency Impairs Contractility and Metabolic Homeostasis in Hypertension.
Altara R, et al.
Frontiers in Pharmacology, 7, 436-436 (2016)
Ivan Kushkevych et al.
Journal of clinical medicine, 8(7) (2019-07-25)
The small-large intestine axis in hydrogen sulfide accumulation and testing of sulfate and lactate in the gut-gut axis of the intestinal environment has not been well described. Sulfate reducing bacteria (SRB) of the Desulfovibrio genus reduce sulfate to hydrogen sulfide
Annasofia Anemone et al.
International journal of oncology, 51(2), 498-506 (2017-07-18)
Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo
Juping Zhao et al.
Molecular medicine reports, 16(6), 8335-8344 (2017-10-07)
Previous studies have indicated that high expression of lactate dehydrogenase A (LDHA) exists in many human cancers. Recently, several reports showed that silencing or inhibition of LDHA could suppress metastasis of human cancer including renal cell carcinoma (RCC). However, the
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Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

需氧糖酵解和瓦博格效应

本页面介绍了一篇有关瓦博格效应的文章，以及其如何能够在正常氧气水平下，增强肿瘤细胞中葡萄糖向乳酸的转化。Otto Heinrich Warburg在1924年证明，癌细胞显示出对糖酵解的依赖性增加，以满足他们的能量需求，无论是否有充足的氧气存在。

Related Content

Global Trade Item Number

SKUGTIN
MAK064-1KT04061835513864

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