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Merck
CN

MAK075

Ascorbic Acid Assay Kit II

sufficient for 100 colorimetric tests

Synonym(s):

FRASC Assay, Ferric Reducing/Antioxidant and Ascorbic Acid

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

General description

The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Application

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.
Ascorbic Acid Assay Kit II has been used to determine ascorbic acid concentration in vitreous sample from the eye.

Biochem/physiol Actions

In this assay, ascorbic acid concentration is determined using the Ferric Reducing/Antioxidant and Ascorbic Acid (FRASC) assay. Fe3+ is reduced to Fe2+ by antioxidants present in the sample, which results in a colorimetric (593 nm) product. The addition of ascorbate oxidase to parallel samples oxidizes any ascorbic acid present allowing for the measurement of the ascorbic acid concentration.


Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Information

监管及禁止进口产品

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Global Trade Item Number

SKUGTIN
MAK075-1KT04061834065111