usage
sufficient for 500 fluorometric tests
detection method
fluorometric
storage temp.
−20°C
Gene Information
human ... ACP1(52), ACP2(53)
mouse ... ACP1(11431), ACP2(11432)
rat ... ACP1(24161), ACP2(24162)
General description
The new Acid Phosphatase Fluorometric Assay Kit, MAK525, is now available! Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.
Application
Suitable for the detection of acid phosphatase in serum and other biological samples.
Features and Benefits
Compatible with high-throughput handling systems.
Principle
The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.
Storage Class Code
12 - Non Combustible Liquids
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