MAK087
Acid Phosphatase Activity Fluorometric Assay Kit
sufficient for 500 fluorometric tests
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About This Item
EC Number:
231-834-5
UNSPSC Code:
12352200
usage
sufficient for 500 fluorometric tests
detection method
fluorometric
storage temp.
−20°C
Gene Information
human ... ACP1(52), ACP2(53)
mouse ... ACP1(11431), ACP2(11432)
rat ... ACP1(24161), ACP2(24162)
General description
The new Acid Phosphatase Fluorometric Assay Kit, MAK525, is now available! Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.
Application
Suitable for the detection of acid phosphatase in serum and other biological samples.
Biochem/physiol Actions
The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.
Features and Benefits
Compatible with high-throughput handling systems.
Storage Class
12 - Non Combustible Liquids
Regulatory Information
监管及禁止进口产品
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Related Content
Instructions
Significance of increased phosphatase activity of bone at the site of osteoplastic metastases secondary to carcinoma of the prostate gland.
Gutman E B, et al.
Cancer Research, 28(3), 485-495 (1936)
Kapil D Patel et al.
ACS applied bio materials, 2(11), 5190-5203 (2019-11-18)
Providing appropriate biophysical and biochemical cues to the interface is a facile strategy to enhance the osteogenic ability of metallic implants. Here we exploited this through the incorporation of mesoporous bioactive glass nanoparticles (MBGN) at a high content (1:1 by
Ruibin Kuang et al.
Plant physiology, 151(1), 199-209 (2009-07-28)
Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAK087-1KT | 04061826727805 |