MAK102
PEP Colorimetric/Fluorometric Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Phospho(enol)pyruvate Colorimetric/Fluorometric Assay Kit
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About This Item
UNSPSC Code:
12161503
EC Number:
215-185-5
NACRES:
NA.84
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
Phospho(enol)pyruvate (PEP) in an intermediate in carbohydrate metabolism involved in glycolysis and gluconeogeneis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.
Application
PEP Colorimetric/Fluorometric Assay Kit has been used to measure the concentrations of phosphoenolpyruvate.
Suitable for the measurement of phosphoenolpyruvate (PEP) in tissue, cells and plant extracts
Biochem/physiol Actions
PEP concentration is determined by a coupled enzyme assay, in which PEP is converted to ATP and pyruvate, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PEP present.
Features and Benefits
Compatible with high-throughput handling systems.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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