MAK188
Acid Smase Activity Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
ASMase Activity Assay Kit, Acid Sphingomyelinase Activity Assay Kit
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SMPD1(6609)
mouse ... SMPD1(20597)
rat ... SMPD1(308909)
General description
Acid Sphingomyelinase (ASMase) is a key enzyme in the sphingolipid pathway that hydrolyzes sphingomyelin to phosphorylcholine and ceramide. Ceramide acts as a lipid second messenger and plays a crucial role in diseases such as cystic fibrosis, diabetes, cancer, and Alzheimer′s disease.
The Acid Sphingomyelinase Activity Colorimetric Assay Kit is a simple and high throughput adaptable assay for measuring ASMase activity in a variety of tissues and cells. ASMase activity is determined by measuring a colorimetric product with absorbance at 570 nm (A570) proportional to the enzymatic activity present. One unit of acid sphingomyelinase is the amount of enzyme required to generate 1.0 μmole of choline per minute at pH 5.0 at 37 °C.
The Acid Sphingomyelinase Activity Colorimetric Assay Kit is a simple and high throughput adaptable assay for measuring ASMase activity in a variety of tissues and cells. ASMase activity is determined by measuring a colorimetric product with absorbance at 570 nm (A570) proportional to the enzymatic activity present. One unit of acid sphingomyelinase is the amount of enzyme required to generate 1.0 μmole of choline per minute at pH 5.0 at 37 °C.
Application
This kit is suitable for the detection of ASMase activity in samples such as animal tissue, cell culture, and serum samples.
Features and Benefits
Compatible with high-throughput liquid handling systems.
Kit Components Only
Product No.
Description
- ASMase Assay Buffer I
- ASMase Assay Buffer II
- ASMase Substrate
- ASMase Enzyme Mix I
- ASMase Enzyme Mix II
- Choline Standard
- ASMase Positive Control
- ASMase Probe, in DMSO
See All (8)
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service