MAK192
Cholesterol Efflux Assay Kit
Sufficient for 100 Fluorometric tests
application(s)
cosmetics
food and beverages
pharmaceutical
detection method
fluorometric
relevant disease(s)
cardiovascular diseases
storage temp.
−20°C
General description
Reverse Cholesterol Transport (RCT) is the mechanism by which the peripheral lipid burden is reduced by transport to liver and its fecal excretion. The first important step in RCT is the cholesterol efflux from macrophage-derived foam cells present in atherosclerotic plaques. The cholesterol efflux is critical for the maintenance of net cholesterol balance in arterial walls and in the reduction of proinflammatory responses triggered by lipid-laden macrophages.
Application
Cholesterol Efflux Assay Kit has been used in cholesterol efflux assay.
Suitable for the determination of cholesterol efflux in cells.
Biochem/physiol Actions
The Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol (λEx = 482 nm/ λEm = 515 nm). It is suitable for serum, isolated, or recombinant lipoprotein samples. It may also be used to screen small molecules for their effect on cholesterol efflux and therefore, may be a valuable tool in drug discovery programs.
Features and Benefits
Compatible with high-throughput handling systems.
Kit Components Only
Product No.
Description
- Labeling Reagent
- Equilibration Buffer
- Reagent A
- Reagent B
- Cell Lysis Buffer
- Positive Control
- Serum Treatment Reagent
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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Knut Tore Lappegård et al.
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 57(1), 91-96 (2018-02-06)
Lipoprotein apheresis and proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors are last therapeutic resorts in patients with familial hypercholesterolemia (FH). We explored changes in lipoprotein subclasses and high-density lipoprotein (HDL) function when changing treatment from lipoprotein apheresis to PCSK9 inhibition.
Sustained delivery of BSA/HSA from biocompatible plant cellulose nanocrystals for in vitro cholesterol release from endothelial cells.
Singla R, et al.
International Journal of Biological Macromolecules, 104, 748-757 (2017)
Xinpeng Chen et al.
Journal of cellular physiology, 236(6), 4750-4763 (2021-02-23)
Excessively high cholesterol content in the blood leads to nonalcohol fatty liver disease (NAFLD) and arteriosclerosis. Although there are increasing publications and patent applications to lower blood cholesterol with small chemical molecules, limited effective drugs can be available in clinic. It
Anastasia Darwitan et al.
Advanced healthcare materials, 9(14), e2000465-e2000465 (2020-06-17)
Atherosclerosis is a chronic disease that can lead to life-threatening events such as myocardial infarction and stroke, is characterized by the build-up of lipids and immune cells within the arterial wall. It is understood that inflammation is a hallmark of
Marc Clément et al.
Circulation, 134(14), 1039-1051 (2016-09-03)
Atherosclerotic lesion expansion is characterized by the development of a lipid-rich necrotic core known to be associated with the occurrence of complications. Abnormal lipid handling, inflammation, and alteration of cell survival or proliferation contribute to necrotic core formation, but the
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