MAK193
Citrate Synthase Activity Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
Citrate Synthase Test Kit
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About This Item
UNSPSC Code:
12161503
EC Number:
232-821-7
NACRES:
NA.84
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
Gene Information
human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)
General description
Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.
Application
Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.
Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Biochem/physiol Actions
The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.
Kit Components Only
Product No.
Description
- CS Assay Buffer
- CS Substrate Mix
- CS Developer
- GSH Standard, Reduced
- CS Positive Control
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
低风险生物材料
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Md Habibur Rahman et al.
Microsystems & nanoengineering, 4, 39-39 (2019-05-07)
Defective mitochondria have been linked to several critical human diseases such as neurodegenerative disorders, cancers and cardiovascular disease. However, the detailed characterization of mitochondria has remained relatively unexplored, largely due to the lack of effective extraction methods that may sufficiently
Xi Lin et al.
International journal of molecular sciences, 21(3) (2020-01-26)
Maintaining an active fatty acid metabolism is important for renal growth, development, and health. We evaluated the effects of anaplerotic and ketogenic energy sources on fatty acid oxidation during stimulation with clofibrate, a pharmacologic peroxisome proliferator-activated receptor α (PPARα) agonist.
Tony Chao et al.
American journal of physiology. Cell physiology, 317(6), C1229-C1238 (2019-09-19)
Severe burn injury results in systemic disruption of metabolic regulations and impaired cardiac function. Restoration of hemodynamic homeostasis utilizing intravenous (IV) fluids is critical for acute care of the burn victim. However, the effects of burns and resuscitation on cardiomyocyte
Regulation of Expression of Citrate Synthase by the Retinoic Acid Receptor-Related Orphan Receptor α (RORα)
Crumbley C, et al.,
PLoS ONE, 7, e33804 -e33804 (2012)
P Dongil et al.
Scientific reports, 8(1), 13810-13810 (2018-09-16)
Metabolic dysfunction in the liver is the cause of numerous pathologies, which are associated with an altered redox state. PASK (PAS Domain Kinase) is a nutrient and bioenergetic sensor. We contend that PASK could act as an oxidative stress sensor
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