MAK197
Succinate Dehydrogenase Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
SDH Activity Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
detection method
colorimetric
relevant disease(s)
neurological disorders
storage temp.
−20°C
Gene Information
human ... SDHA(6389), SDHAF1(644096), SDHAF2(54949), SDHB(6390), SDHC(6391), SDHD(6392)
mouse ... SDHA(66945), SDHAF1(68332), SDHAF2(66072), SDHB(67680), SDHC(66052), SDHD(66925)
rat ... SDHA(157074), SDHAF2(361726), SDHB(298596), SDHC(289217), SDHD(363061)
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General description
Succinate dehydrogenase (SDH; EC 1.3.5.1) is a mitochondrial enzyme that catalyzes the oxidation of succinate to fumarate and carries electrons from FADH to CoQ in eukaryotes and bacteria. It has a central function in the maintenance of cellular energy metabolism via the Krebs (tricarboxylic acid) cycle and the electron transport chain. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome.
Application
Succinate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of succinate dehydrogenase in samples.
Suitable for the detection of Succinate Dehydrogenase Activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Biochem/physiol Actions
The Succinate Dehydrogenase Activity Colorimetric Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.
Kit Components Only
Product No.
Description
- SDH Assay Buffer
- SDH Substrate Mix
- SDH Probe
- DCIP Standard, 2 mM
- SDH Positive Control
Storage Class
10 - Combustible liquids
Regulatory Information
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