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MAK199

Sigma-Aldrich

Acetoacetate Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

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Synonym(s):
AcAc Assay Kit
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

General description

Acetoacetate (AcAc) is a β-ketoacid formed in the mitochondria by the condensation of two molecules of acetyl-CoA. AcAc is either enzymatically reduced to 3-hydroxybutyrate or decarboxylated to produce acetone. Ketone bodies (AcAc, 3-HB, and acetone) are used as alternate source of energy in the absence of glucose. Excess ketone bodies are observed in type I diabetes, alcoholism, or starvation.

Suitability

Suitable for the detection of acetoacetate in samples such as serum, plasma, blood, urine, and other body fluids.

Principle

The Acetoacetate Colorimetric Assay kit provides a simple and sensitive procedure for measuring AcAc in various samples (ranging from 20-100 nmole/well). AcAc concentration is determined by generating a colorimetric (550 nm) product proportional to the amount of AcAc present. The kit does not detect 3-hydroxybutyrate and is specific for acetoacetate.

Kit Components Only

Product No.
Description
  • Acetoacetate Assay Buffer
  • Acetoacetate Standard
  • Acetoacetate Substrate

replaced by

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Information

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Anna Go et al.
Sensors (Basel, Switzerland), 21(14) (2021-07-25)
In this report, we present an enzyme deposited Au electrode for an electrochemical measurement of acetylacetic acid (AcAc) in urine. The electrode has an immobilized layer of a mixture of D-β-hydroxybutyrate dehydrogenase (HBDH) and nicotinamide adenine dinucleotide (NADH) as sensing
Tae Woong Whon et al.
EMBO reports, 22(1), e50663-e50663 (2020-11-24)
Castration of young males is widely used in the cattle industry to improve meat quality, but the mechanism linking hypogonadism and host metabolism is not clear. Here, we use metataxonomic and metabolomic approaches to evaluate the intestinal microbiota and host
Daniela Liśkiewicz et al.
Frontiers in cellular neuroscience, 15, 733607-733607 (2021-08-31)
Experimental and clinical data support the neuroprotective properties of the ketogenic diet and ketone bodies, but there is still a lot to discover to comprehensively understand the underlying mechanisms. Autophagy is a key mechanism for maintaining cell homeostasis, and therefore
Enrique Chávez et al.
The Journal of pharmacology and experimental therapeutics, 361(2), 292-302 (2017-02-18)
Background: Mitochondrion is an important metabolic and energetic organelle that regulates several cellular processes. Mitochondrial dysfunction has been related to liver diseases including hepatocellular carcinoma. As a result, the energetic demand is not properly supplied and mitochondrial morphologic changes have

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