MAK217
Succinyl-CoA Synthetase Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
Succinate Thiokinase Assay Kit, succinyl-CoA ligase Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352200
usage
sufficient for 100 reactions (Colorimetric)
detection method
colorimetric
storage temp.
−20°C
General description
Succinyl-CoA synthetase (SCS; succinyl-CoA ligase; succinate thiokinase; EC 6.2.1.5), also known as succinyl-CoA synthetase ligase/succinate thiokinase, is a mitochondrial enzyme. The enzyme catalyzes a substrate-level phosphorylation of GDP or ADP and thus, plays a vital role in Krebs cycle. In addition, it also catalyzes the reverse reaction to supply succinyl-CoA for heme synthesis and ketone body activation. Deficiency in SCS activity is associated with encephalomyopathy and depletion of mitochondrial DNA.Aberrations in the gene expression leads to the development of diabetes and neurodegenerative diseases.
Application
Suitable for the detection of Succinly-CoA synthetase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Biochem/physiol Actions
The Succinyl-CoA Synthetase Activity Colorimetric Assay Kit is a simple and high throughput adaptable assay for measuring SCS activity in a variety of tissues, cells, and isolated mitochondria. SCS activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of succinyl-CoA synthetase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.4 at 25 °C.
Features and Benefits
Compatible with high-throughput handling systems.
Kit Components Only
Product No.
Description
- SCS Assay Buffer
- SCS Substrate Mix
- SCS Enzyme Mix
- SCS Developer
- NADH Standard
- SCS Positive Control
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
Regulatory Information
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Characterization of the ATP-and GTP-specific Succinyl-CoA Synthetases in Pigeon THE ENZYMES INCORPORATE THE SAME α-SUBUNIT.
Johnson, J. D,
The Journal of Biological Chemistry, 273(42), 27573-27579 (1998)
Deficiency of the ADP-Forming Succinyl-CoA Synthase Activity Is Associated with Encephalomyopathy and Mitochondrial DNA Depletion
Orly E, et al.
American Journal of Human Genetics, 76(6), 1081-1086 (2005)
Abby J Chiang et al.
mSystems, 4(4) (2019-06-13)
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