MAK221
Beta-Lactamase Activity Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
β-Lactamase Activity Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
detection method
colorimetric
storage temp.
−20°C
General description
β-Lactamase (bL; EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of βLs have since been identified from various bacteria. βLs specifically hydrolyze β-lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.
Application
Suitable for the detection of β-lactamase activity in biological samples, food, and fermentation samples.
Biochem/physiol Actions
The β-Lactamase Activity Assay Kit provides a simple assay for measuring βL activity in βL-secreting bacteria in fermentation media and bacterial cultures. It can also be used to detect βL-secreting bacteria in saliva, urine, and serum of infected mammals and in food samples. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present. One unit of β-lactamase is the amount of enzyme required to hydrolyze 1.0 μmole of nitrocefin per minute at pH 7.0 at 25 °C.
Kit Components Only
Product No.
Description
- β-Lactamase Assay Buffer
- Nitrocefin, in DMSO
- Positive Control
- βL Hydrolysis Buffer
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
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Jennifer M Colquhoun et al.
mBio, 12(6), e0313721-e0313721 (2021-12-08)
β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii. In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up
Class C β-lactamases operate at the diffusion limit for turnover of their preferred cephalosporin substrates.
Bulychev A and Shahriar M
Antimicrobial Agents and Chemotherapy, 43(7), 1743-1746 (1999)
Beta-lactam antibiotics: from antibiosis to resistance and bacteriology.
Kong K F, et al.
Acta Pathologica, Microbiologica et Immunological Scandinavica, 118(1), 1-36 (2010)
A S Gargis et al.
mSystems, 3(6) (2018-12-24)
Penicillin (PEN) is a low-cost option for anthrax treatment, but naturally occurring resistance has been reported. β-Lactamase expression (bla1, bla2) in Bacillus anthracis is regulated by a sigma factor (SigP) and its cognate anti-sigma factor (RsiP). Mutations leading to truncation
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