High Sensitivity DHAP Fluorometric Assay Kit

Dihydroxyacetone phosphate (DHAP) is a metabolic intermediate involved in many pathways, including glycolysis, gluconeogenesis, glycerol metabolism, phosphatidic acid synthesis, fat metabolism and the Calvin cycle. Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by triose phosphate isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.

Dihydroxyacetone phosphate (DHAP) is a metabolic intermediate involved in many pathways, including glycolysis, gluconeogenesis, glycerol metabolism, phosphatidic acid synthesis, fat metabolism, and the Calvin cycle. Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.

Suitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.

The High Sensitivity Dihydroxyacetone Phosphate Assay Kit is suitable for measuring low levels of DHAP. In this assay, TPI converts DHAP to GAP producing a coupled enzyme reaction, which results in a fluorometric product (lex = 535 nm/lem = 587 nm), proportional to the amount of dihydroxyacetone phosphate present. This assay kit is simple and sensitive with the capability to detect to 0.5 mM of dihydroxyacetone phosphate in a variety of samples.