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Merck
CN

MAK294

Sigma-Aldrich

MMP-3 Inhibitor Screening Kit (Fluorometric)

sufficient for 100 fluorometric tests

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25
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detection method

fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Similar to several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including Ecadherin, a known contributor to cancer development.

Application

Suitable to screen inhibitors of MMP-3.

Biochem/physiol Actions

In the MMP-3 Inhibitor Screening Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically ( λex = 325nm/λem = 393nm). The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of MMP-3 inhibition.

Features and Benefits

Compatible with high-throughput handling systems.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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