MAK363
Lipoxygenase Activity Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
LOX Enzyme Activity Detection Kit
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
General description
Lipoxygenases (LOX) are non heme iron-containing dioxygenases. These enzymes convert unsaturated fatty acids to epoxides; for example, the synthesis of leukotrienes from arachidonic acid. They play an important role in cell proliferation, differentiation and inflammation; and have been implicated in inflammation and hyper proliferation mediated diseases like asthma, rheumatoid arthritis and cancer.
Application
Suitable for the measurement of lipoxygenase activity in cell and tissue lysates, biological fluids (eg. serum), recombinant enzyme and purified protein.
Biochem/physiol Actions
In the Lipoxygenase Activity Assay Kit, lipoxygenase converts the LOX substrate to an intermediate that reacts with the probe generating a fluorescent product. The increase in fluorescent signal can be recorded at λex = 500 nm/λem = 536 nm and is directly proportional to LOX activity. The kit includes 5-lipoxygenase enzyme (LOX Enzyme) as a positive control. A lipoxygenase inhibitor that completely inhibits lipoxygenase activity is also included in order to calculate the specific activity of LOX in biological samples. The kit can detect as low as 0.004 mU/mg protein.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1
Storage Class Code
3 - Flammable liquids
Flash Point(F)
49.5 °F
Flash Point(C)
9.7 °C
Regulatory Information
动植物源性产品
危险化学品
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