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Merck
CN

MAK405

Sigma-Aldrich

Lysosome Isolation Kit

sufficient for 50 extractions

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application(s)

sample preservation

storage temp.

−20°C

General description

Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm.

Application

The kit is suitable for the isolation of high purity lysosomes from mammalian tissues (liver, kidney, etc.) and cultured cells (suspension and adherent cells).

Biochem/physiol Actions

The Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.

Features and Benefits

Lysosome Isolation Kit:
  • Provides unique formulations of ready-to-use buffers and reagents to isolate lysosomes with minimal sample preparation.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Information

常规特殊物品
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