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Merck
CN

MAK478

Sigma-Aldrich

α-Amylase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Assay Kit for Amylase Activity

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.84
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Quality Level

application(s)

agriculture
food and beverages

detection method

colorimetric

relevant disease(s)

endocrinological disorders; gastrointestinal diseases; genitourinary disorders

shipped in

wet ice

storage temp.

−20°C

General description

Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4- glycosidic bonds. The α-amylases cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis, and renal failure.

Application

The kit is suitable for the determination of α-amylase activity in blood, saliva, urine, grains, and other agricultural samples.

Biochem/physiol Actions

Simple, direct, and automation-ready procedures for measuring amylase activity are very desirable. The α-Amylase Activity Assay Kit method involves two steps. First, α -amylase in the sample hydrolyses starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase. Next, the hydrogen peroxide concentration is determined with a colorimetric reagent at 585 nm.

Features and Benefits

  • The linear detection range of the kit is 0.3 to 50 U/L α-amylase.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Information

含少量动物源组分生物产品
常规特殊物品
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