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Merck
CN

MCL1

Mammalian Cell Lysis Kit

Synonym(s):

Cell Lysis Reagent

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About This Item

NACRES:
NA.56
UNSPSC Code:
12352200
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Quality Level

usage

 kit sufficient for 250 extractions (100 mm tissue culture plates)

technique(s)

immunoprecipitation (IP): suitable

shipped in

dry ice

storage temp.

−20°C

Application

The product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.

Features and Benefits

  • The kit can be used to prepare cell lysis buffer and other modified buffers
  • The kit enables the researcher to change the buffer′s components to obtain maximum efficiency for a specific protein

Other Notes

The mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • Protease inhibitor cocktail 2.5 mL
    SDS

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 1

pictograms

CorrosionEnvironment

signalword

Danger

Regulatory Information

常规特殊物品

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Shintaro Nakao et al.
Investigative ophthalmology & visual science, 54(6), 3830-3836 (2013-04-13)
Inflammation and immune cells regulate choroidal neovascularization (CNV) and could become therapeutic targets in age-related macular degeneration (AMD). Lymphangiogenesis is a key component of various inflammatory diseases. Whether lymphangiogenesis and lymph node-mediated immunity are involved in the pathogenesis of AMD
Shintaro Nakao et al.
Blood, 117(3), 1081-1090 (2010-08-14)
Angio- and lymphangiogenesis are inherently related processes. However, how blood and lymphatic vessels regulate each other is unknown. This work introduces a novel mechanism explaining the temporal and spatial relation of blood and lymphatic vessels. Vascular endothelial growth factor-A (VEGF-A)
Shinsuke Yasuda et al.
Journal of immunology (Baltimore, Md. : 1950), 179(7), 4890-4900 (2007-09-20)
Dysregulation of Ras guanyl nucleotide-releasing protein 1 (RasGRP1) in mice results in a systemic lupus erythematosus (SLE)-like disorder. We therefore looked for defective isoforms and/or diminished levels of human RasGRP1 in a cohort of SLE patients. PBMCs were collected from



Global Trade Item Number

SKUGTIN
APREST70112-100UL04061835937011
APREST70113-100UL04061835937028
SAB5701110-100UL04065267662879
SAB4501843-100UG04061837188336
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AMAB90859-100UL04061837048258
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SAB5700554-100UL04065265176996
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EHU079921-50UG04061831373356