MD0100
MGMT Assay Kit
sufficient for 100 tests
Synonym(s):
O6-Methylguanine-DNA Methyltransferase Assay Kit
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About This Item
UNSPSC Code:
12352200
usage
sufficient for 100 tests
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MGMT(4255)
Application
MGMT (O6-Methylguanine-DNA methyltransferase) is a ubiquitous DNA repair protein that removes O6-alkyl-guanine lesions, primarily O6-methyl-guanine from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation.
The MGMT assay kit is based on the formation of a new restriction site in a 23-mer 32P labeled ds-oligonucleotide after removal of the O6-methyl-guanine lesion. Cleavage of the new restriction site results in two smaller oligonucleotides that can be resolved on a denaturing PAGE gel.
The MGMT assay kit is based on the formation of a new restriction site in a 23-mer 32P labeled ds-oligonucleotide after removal of the O6-methyl-guanine lesion. Cleavage of the new restriction site results in two smaller oligonucleotides that can be resolved on a denaturing PAGE gel.
Features and Benefits
The kit can be used for:
- Screening for MGMT inhibitor.
- Detection of MGMT activity in cell lines.
Kit Components Only
Product No.
Description
- MGMT substrate O6-methylguanine strand 1 mL/vial
- MGMT substrate complementary strand 1 mL/vial
- Reaction buffer 10X 1.5 mL
- Stop solution 1 mL
- Methylguanine DNA methyltransferase (MGMT) 10 μg
- O6Benzylguanine solution 50 μL
- Spin column-10 2 U
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W K Hansen et al.
The Journal of pharmacology and experimental therapeutics, 295(1), 1-9 (2000-09-19)
The area of mammalian DNA repair and its relationship to cancer and therapeutic approaches is rapidly growing, both through the studies of basic mechanisms and in the use of this knowledge for translational applications. We have attempted to briefly and
M Sassanfar et al.
The Journal of biological chemistry, 266(5), 2767-2771 (1991-02-15)
The suicidal inactivation mechanism of DNA repair methyltransferases (MTases) was exploited to measure the relative efficiencies with which the Escherichia coli, human, and Saccharomyces cerevisiae DNA MTases repair O6-methylguanine (O6MeG) and O4-methylthymine (O4MeT), two of the DNA lesions produced by
Geoffrey P Margison et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 24(3), 255-266 (2002-03-14)
The DNA in human cells is continuously undergoing damage as consequences of both endogenous processes and exposure to exogenous agents. The resulting structural changes can be repaired by a number of systems that function to preserve genome integrity. Most pathways
L P Encell et al.
Biochemistry, 38(37), 12097-12103 (1999-10-03)
Human O(6)-alkylguanine-DNA alkyltransferase (MGMT) repairs potentially cytotoxic and mutagenic alkylation damage at the O(6)-position of guanine and the O(4)-position of thymine in DNA. We have used random sequence mutagenesis and functional complementation to obtain human MGMT mutants that are resistant
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