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MP0025

Venor™ GeM Mycoplasma Detection Kit, PCR-based

Synonym(s):

PCR mycoplasma detection kit

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
Application(s):
detection
microbiology
Technique(s):
PCR: suitable
Storage temp.:
2-8°C
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usage

 kit sufficient for 25 tests

Quality Level

200

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

Related Categories

General description

The Venor™ GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.

Application

The PCR-based Venor™ GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.

Preparation Note

The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.

Other Notes

Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase

Legal Information

Venor is a trademark of Minerva Biolabs GmbH

Kit Components Only

Product No.
Description
  • Positive Control
  • Negative Control
  • PCR 10X Reaction Buffer 500 mL/vial
  • Primer/Nucleotide Mix 1 mL/vial

Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000483162
0000483162
0000406903
0000406903
0000353055

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Julie V Philley et al.
Journal of cellular physiology, 231(6), 1364-1374 (2015-11-05)
Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa
Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management
Russell BJ, et al.
Journal of Virological Methods (2020)
Tomohiro Aoki et al.
Cancer discovery, 10(3), 406-421 (2019-12-21)
Hodgkin lymphoma is characterized by an extensively dominant tumor microenvironment (TME) composed of different types of noncancerous immune cells with rare malignant cells. Characterization of the cellular components and their spatial relationship is crucial to understanding cross-talk and therapeutic targeting
Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state
Kherdjemil Y, et al.
Nature (2016)
Anbarasu Kannan et al.
Scientific reports, 7, 46102-46102 (2017-04-07)
Human papilloma virus-16 (HPV-16) associated oropharyngeal cancer (HPVOPC) is increasing alarmingly in the United States. We performed whole genome sequencing of a 44 year old, male HPVOPC subject diagnosed with moderately differentiated tonsillar carcinoma. We identified new somatic mutation in
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Global Trade Item Number

SKUGTIN
MP0025-1KT04061834066811

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