MP0040
LookOut® mycoplasma qPCR detection kit
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About This Item
EC Number:
UNSPSC Code:
12352200
usage
sufficient for 25 reactions
quality
Not suitable for clinical diagnostic use.
technique(s)
PCR: suitable
application(s)
detection
microbiology
storage temp.
2-8°C
General description
The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.
The primer/probe system detects the highly conserved 16S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.
This kit has been tested and optimized for use with JumpStartä Taq DNA Polymerase, Catalog Number D9307. It is highly recommended that this product be used with the kit. Use of other Taq polymerase products may require reaction optimization to achieve proper results.
The primer/probe system detects the highly conserved 16S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.
This kit has been tested and optimized for use with JumpStartä Taq DNA Polymerase, Catalog Number D9307. It is highly recommended that this product be used with the kit. Use of other Taq polymerase products may require reaction optimization to achieve proper results.
Application
qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.
Legal Information
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Regulatory Information
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