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About This Item
Empirical Formula (Hill Notation):
C12H15NO8
CAS Number:
Molecular Weight:
301.25
Beilstein:
1436646
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
Product Name
4-Nitrophenyl β-D-mannopyranoside, ≥98%
Quality Level
Assay
≥98%
form
powder
solubility
water: 10 mg/mL, clear, colorless to very faintly green
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2ccc(cc2)[N+]([O-])=O)[C@@H](O)[C@@H](O)[C@@H]1O
InChI
1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11+,12-/m1/s1
InChI key
IFBHRQDFSNCLOZ-LDMBFOFVSA-N
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Application
4-Nitrophenyl β-D-mannopyranoside has been used:
- as a substrate to analyze the action mechanism of Pyrococcus furiosus thermostable glycosidase (PFTG) and kinetic parameters by isothermal titration calorimetry (ITC)
- as a substrate for GH1-glucosidase (EaBgl1A) enzyme
- as a substrate for screening cucumber enzymes
Biochem/physiol Actions
4-Nitrophenyl β-D-mannopyranoside or p-Nitrophenyl-β-D-mannopyranoside is a substrate for determining the activity of β-D-mannopyranosidase, an enzyme required to study about glycoproteins structural details.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
涉药品监管产品
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Evaluation of enzymatic and non-enzymatic softening in low salt cucumber fermentations
Maruvada R and McFeeters RF
International Journal of Food Science and Technology, 44(6), 1108-1117 (2009)
G W Forsyth et al.
Clinica chimica acta; international journal of clinical chemistry, 216(1-2), 11-21 (1993-07-16)
The cytosolic beta-glucosidase activity that is found in a variety of mammalian tissues has no clearly defined function. In vitro assay conditions under which the broad-specificity beta-glucosidase hydrolyzes glucocerebroside at a significant rate have not been described. Nonetheless, it has
K Cavanagh et al.
American journal of veterinary research, 44(4), 681-684 (1983-04-01)
Experimental conditions for measuring caprine plasma beta-D-mannosidase activity are described with p-nitrophenyl-beta-D-mannopyranoside as a substrate. The plasma enzyme was stable for 3 months at -20 C or 1 week at 4 C. The optimal pH for activity was 5.0 in
Tanin Tangkuaram et al.
The Analyst, 131(8), 889-891 (2006-10-10)
Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced
Yunhan Hsu et al.
Fungal genetics and biology : FG & B, 144, 103441-103441 (2020-08-11)
To better understand the roles of genes involved in mannan degradation in filamentous fungi, in this study we searched, identified, and characterized one putative GH5 endo-β-mannanase (GH5-7) and two putative GH2 mannan-degrading enzymes (GH2-1 and GH2-4) in Neurospora crassa. Real-time
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