recombinant
expressed in baculovirus infected Sf9 cells
form
buffered aqueous solution
specific activity
≥0.3 units/mg protein
mol wt
135 (kDa/subunit, homodimer)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
cow ... NOS3(287024)
Biochem/physiol Actions
For measuring NOS activity in the presence of exogenously added co-factors and/or inhibitors.
NOS is responsible for the biosynthesis of nitric oxide from L-arginine and is constituitively expressed in endothelial cells (eNOS) and brain (nNOS).
Responsible for the biosynthesis of nitric oxide from L-arginine; constituitively expressed in endothelial cells (eNOS) and brain (nNOS).
Suitable for measuring NOS activity in the presence of exogenously added co-factors and/or inhibitors.
Unit Definition
One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.
Physical form
Solution in 50 mM HEPES, pH 7.4, with 10% glycerol, 5 mM CHAPS, and 100 μM DTT.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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