N1533
Nitric Oxide Synthase, Endothelial bovine
recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous solution
Synonym(s):
NOS III, ecNOS
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About This Item
recombinant
expressed in baculovirus infected Sf9 cells
form
buffered aqueous solution
specific activity
≥0.3 units/mg protein
mol wt
135 (kDa/subunit, homodimer)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
cow ... NOS3(287024)
Biochem/physiol Actions
For measuring NOS activity in the presence of exogenously added co-factors and/or inhibitors.
NOS is responsible for the biosynthesis of nitric oxide from L-arginine and is constituitively expressed in endothelial cells (eNOS) and brain (nNOS).
Responsible for the biosynthesis of nitric oxide from L-arginine; constituitively expressed in endothelial cells (eNOS) and brain (nNOS).
Suitable for measuring NOS activity in the presence of exogenously added co-factors and/or inhibitors.
Physical form
Solution in 50 mM HEPES, pH 7.4, with 10% glycerol, 5 mM CHAPS, and 100 μM DTT.
Other Notes
One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Related Content
Datasheet
Inhibition of L-arginine metabolizing enzymes by L-arginine-derived advanced glycation end products.
Ying-Ling Lai et al.
Journal of clinical biochemistry and nutrition, 46(2), 177-185 (2010-03-11)
N(omega)-Carboxymethyl-arginine (CMA), N(omega)-carboxyethyl-arginine (CEA) and N(delta)-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1) have been identified as L-arginine-derived advanced glycation end products (AGEs) formed by non-enzymatic reactions between reducing sugars such as glucose and amino groups in proteins. These AGEs are structurally analogous to endogenous inhibitors
Ying-Ling Lai et al.
Journal of clinical biochemistry and nutrition, 50(1), 84-89 (2012-01-17)
The cholesterol ozonolysis products secosterol-A and its aldolization product secosterol-B were recently detected in human atherosclerotic tissues and brain specimens, and have been postulated to play pivotal roles in the pathogenesis of atherosclerosis and neurodegenerative diseases. We examined several oxidized
Silviya R Stateva et al.
PloS one, 10(4), e0120798-e0120798 (2015-04-02)
Calmodulin (CaM) phosphorylated at different serine/threonine and tyrosine residues is known to exert differential regulatory effects on a variety of CaM-binding enzymes as compared to non-phosphorylated CaM. In this report we describe the preparation and characterization of a series of