N2665
Nucleoside Deoxyribosyltransferase II from Lactobacillus leichmanii
lyophilized powder, recombinant, expressed in E. coli
Synonym(s):
Nucleoside Deoxyribosyltransferase II from Lactobacillus leichmanii, DRTase, Deoxyribose transferase, NDT, nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase
recombinant
expressed in E. coli
assay
≥80% protein basis (biuret)
form
lyophilized powder
specific activity
≥1.0 units/mg protein
sequence note
MPKKTIYFGAGWFTDRQNKAYKEAMEALKENPTIDLENSYVPLDNQYKGIRVDEHPEYLHDKVWATATYNNDLNGIKTNDIMLGVYIPDEEDVGLGMELGYALSQGKYVLLVIPDEDYGKPINLMSWGVSDNVIKMSQLKDFNFNKPRFDFYEGAVY
storage temp.
−20°C
Quality Level
Application
Nucleoside deoxyribosyltransferase II has been used in a study that assessed its enzymatic synthesis with 2′-deoxyguanosine. Nucleoside deoxyribosyltransferase II has also been used in studies to investigate its molecular cloning, expression and specificity.
Suitable for the enzymatic nucleoside synthesis.
Biochem/physiol Actions
Enzymatic nucleoside synthesis is an attractive alternative to traditional chemical methods which require multiple chemical reactions and the use of chemicals that are both expensive and environmentally harmful. Class II Nucleoside Deoxyribosyltransferases exhibit multiple characteristics that make them suitable as biocatalysts for the synthesis of natural and nonnatural nucleosides. They are specific for 2′-deoxyribonucleosides, regioselective (N-1 glycosylation in pyrimidine and N-9 in purine), and stereoselective (forming only β-anomers).
Class II N-Deoxyribosyltranferases, DRTases, catalyze the transfer of a 2′-deoxyribosyl group between purines or pyrimidines. In the absence of an acceptor nucleobase, these enzymes display hydrolase activity, converting the nucleoside to its base and a deoxyribose. In lactobacilli species, Nucleoside Deoxyribosyltransferase enzymes are part of the nucleoside salvage pathway for DNA synthesis.
Class II N-Deoxyribosyltranferases, DRTases, catalyze the transfer of a 2′-deoxyribosyl group between purines or pyrimidines. In the absence of an acceptor nucleobase, these enzymes display hydrolase activity, converting the nucleoside to its base and a deoxyribose. In lactobacilli species, Nucleoside Deoxyribosyltransferase enzymes are part of the nucleoside salvage pathway for DNA synthesis.
Preparation Note
Produced using animal component-free materials.
Other Notes
One unit of enzyme produces 1 μM of hypoxanthine in 1 minute at 40°C, pH 6.0.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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D J Porter et al.
The Journal of biological chemistry, 270(26), 15557-15562 (1995-06-30)
Nucleoside 2-deoxyribosyltransferase catalyzes cleavage of a 2'-deoxyribosylnucleoside (A) to a nucleobase (P) with deoxyribosylation of the enzyme. Substrates quenched the intrinsic fluorescence of native enzyme (E) and a catalytically inactive mutant enzyme (E98A enzyme). The time courses of these reactions
Marcos Almendros et al.
Molecules (Basel, Switzerland), 14(3), 1279-1287 (2009-03-28)
Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases. High productivity values (t =1 hr) were obtained while completely avoiding adenosine-deaminase degradation
K Okuyama et al.
Bioscience, biotechnology, and biochemistry, 64(10), 2243-2245 (2000-12-29)
Nucleoside deoxyribosyltransferase-II, which catalyzes transfer of glycosyl residues from a donor deoxynucleoside to an acceptor base, was purified from Lactobacillus helveticus and its gene was cloned. Analysis of the nucleotide sequence showed the presence of a 474-nucleotide open reading frame
Pierre Alexandre Kaminski et al.
The Journal of biological chemistry, 283(29), 20053-20059 (2008-05-20)
Nucleoside 2'-deoxyribosyltransferases catalyze the transfer of 2-deoxyribose between bases and have been widely used as biocatalysts to synthesize a variety of nucleoside analogs. The genes encoding nucleoside 2'-deoxyribosyltransferase (ndt) from Lactobacillus leichmannii and Lactobacillus fermentum underwent random mutagenesis to select
N C Schnetz-Boutaud et al.
Current protocols in nucleic acid chemistry, Chapter 1, Unit1-Unit1 (2008-04-23)
Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M(1)G-dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl compound malondialdehyde with a dG
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