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Merck
CN

N2752

4-Nitrophenyl palmitate

lipase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

p-Nitrophenyl palmitate, Hexadecanoic acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C22H35NO4
CAS Number:
1492-30-4
Molecular Weight:
377.52
Beilstein:
1891754
EC Number:
216-084-9
MDL number:
MFCD00047732
UNSPSC Code:
12352204
PubChem Substance ID:
24897590
NACRES:
NA.83

Key Documents

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Product Name

4-Nitrophenyl palmitate, lipase substrate

Quality Level

200

Assay

≥98% (TLC)

form

powder

solubility

chloroform: 100 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CCCCCCCCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3

InChI key

LVZSQWIWCANHPF-UHFFFAOYSA-N

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General description

4-Nitrophenyl palmitate is a substrate for lipase enzyme activity. Lipase hydrolyzes 4-nitrophenyl palmitate and yields the yellow colored product 4-nitrophenol, which is measurable spectrophotometrically at 410 nm. This method is advantageous due to its short reaction time and facile spectrophotometric analyses. The cell-bound lipase has preference for 4-nitrophenyl palmitate as substrate than the extracellular lipase.

Application

4-nitrophenyl palmitate has been used as a substrate for lipase enzyme activity.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Precautionary Statements

P280

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000463526
0000463525
0000463525
0000463526
0000460360

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Brabcova J, et al.
Yeast, 27(12), 1029-1038 (2010)
Kai Yuan et al.
Journal of industrial microbiology & biotechnology, 46(8), 1091-1101 (2019-06-21)
Metabolic fluxes during lipase production by Bacillus subtilis CICC 20034 in synthetic medium were studied using metabolic flux analysis (MFA). The MFA showed that lipase production was dependent on, and coupled to the tributyrin uptake rate, formation of biomass, lactate
Seon-Woo Lee et al.
Applied microbiology and biotechnology, 65(6), 720-726 (2004-09-15)
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Herein, as a promising support, a magnetic enzyme nanoformulation have been designed and fabricated by a poly-o-toluidine modification approach. Owing to the magnetic nature and the existence of amine functionalized groups, the as-synthesised poly(o-toluidine) functionalized magnetic nanocomposite (Fe3O4@POT) was employed
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