recombinant
expressed in baculovirus infected Sf9 cells
Assay
≥95%
form
buffered aqueous solution
specific activity
≥200 units/mg protein
mol wt
150 (kDa/subunit, homodimer)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
rat ... Nos1(24598)
Biochem/physiol Actions
NOS is responsible for the biosynthesis of nitric oxide from L-arginine. nNOS is calcium/calmodulin dependent and has a Km = 2.0 μM for L-arginine.
Unit Definition
One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.
Physical form
Solution in 50 mM HEPES, pH 7.4, with 20% glycerol, 100 mM NaCl and 10 μM tetrahydrobiopterin.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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