N3033
Nitric Oxide Synthase, Neuronal from rat
≥95%, recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous solution
Synonym(s):
NOS I, Neuronal Nitric Oxide Synthase, bNOS, ncNOS
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About This Item
UNSPSC Code:
12352200
MDL number:
recombinant
expressed in baculovirus infected Sf9 cells
assay
≥95%
form
buffered aqueous solution
specific activity
≥200 units/mg protein
mol wt
150 (kDa/subunit, homodimer)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
rat ... Nos1(24598)
Biochem/physiol Actions
NOS is responsible for the biosynthesis of nitric oxide from L-arginine. nNOS is calcium/calmodulin dependent and has a Km = 2.0 μM for L-arginine.
Physical form
Solution in 50 mM HEPES, pH 7.4, with 20% glycerol, 100 mM NaCl and 10 μM tetrahydrobiopterin.
Other Notes
One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Darío E Iglesias et al.
Food & function, 10(5), 2528-2537 (2019-04-18)
In order to study the in vitro effect of flavan-3-ol (+)-catechin on the enzymatic activities of mitochondrial complex I and nitric oxide synthase (mtNOS), as well as the consequences on the membrane potential and H2O2 production rate, isolated mitochondria from
Holger Engel et al.
Annals of plastic surgery, 79(4), e25-e29 (2014-04-03)
Nitric oxide (NO) is an important cytoprotective agent against ischemia and reperfusion injury (IRI). Enhancing NO bioavailability via exogen NO synthases (NOSs) and L-arginine promotes conversation to NO, circumventing the problem of nonfunctioning NOSs under hypoxic and acidic conditions. In
Michał Bulc et al.
World journal of gastroenterology, 23(33), 6088-6099 (2017-10-04)
Influence of chronic hyperglycemia on chemical coding of enteric neurons in stomach using pig as a model for human diabetic complications. Ten pigs were divided into two groups: diabetic (D group, In the control group in the myenteric ganglia (MG)
Emilio Varea et al.
Frontiers in neuroscience, 5, 17-17 (2011-03-19)
Neuronal production persists during adulthood in the dentate gyrus and the olfactory bulb, where substantial numbers of immature neurons can be found. These cells can also be found in the paleocortex layer II of adult rodents, but in this case
Emre Gazyakan et al.
Annals of plastic surgery, 84(6), 705-710 (2019-12-19)
Nitric oxide (NO) is a multifunctional signaling molecule involved in regulating vascular tone and tissue oxygenation. It is also an important cytoprotective agent against ischemia-reperfusion injury (IRI). Enhancing NO bioavailability via exogenous NO synthases (NOSs) and L-arginine promotes conversation to
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