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MDL number:
UNSPSC Code:
12352200
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recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥95%

form

buffered aqueous solution

specific activity

≥200 units/mg protein

mol wt

150 (kDa/subunit, homodimer)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

rat ... Nos1(24598)

Biochem/physiol Actions

NOS is responsible for the biosynthesis of nitric oxide from L-arginine. nNOS is calcium/calmodulin dependent and has a Km = 2.0 μM for L-arginine.

Unit Definition

One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.

Physical form

Solution in 50 mM HEPES, pH 7.4, with 20% glycerol, 100 mM NaCl and 10 μM tetrahydrobiopterin.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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