N3254
4-Nitrophenyl phosphate di(tris) salt
phosphatase substrate, ≥97% (enzymatic), powder
Synonym(s):
4-Nitrophenyl phosphate bis(tris) salt
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About This Item
Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
Molecular Weight:
461.36
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
269-198-6
MDL number:
Beilstein/REAXYS Number:
1578142
Product Name
4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate
Quality Segment
assay
≥97% (enzymatic)
form
powder
solubility
water: 50 mg/mL, clear, colorless to light yellow
storage temp.
−20°C
SMILES string
NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key
XXAXKCWOTRABOW-UHFFFAOYSA-N
Application
4-Nitrophenyl phosphate di(tris) salt has been used:
- as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
- to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
- for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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