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N3254

4-Nitrophenyl phosphate di(tris) salt

Name: 4-Nitrophenyl phosphate di(tris) salt
Brand: SIGMA

phosphatase substrate, ≥97% (enzymatic), powder

Synonym(s):

4-Nitrophenyl phosphate bis(tris) salt

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About This Item

Linear Formula:

(C₆ H₄ N O₆ P)( C₄ H₁₂ N O₃)₂

CAS Number:

68189-42-4

Molecular Weight:

461.36

NACRES:

NA.83

PubChem Substance ID:

24897633

UNSPSC Code:

12352204

EC Number:

269-198-6

MDL number:

MFCD00037009

Beilstein/REAXYS Number:

1578142

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Properties

Product Name

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Quality Level

100

assay

≥97% (enzymatic)

form

powder

solubility

water: 50 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

Description

Application

4-Nitrophenyl phosphate di(tris) salt has been used:

as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Global Trade Item Number

SKU	GTIN
N3254-100G	04061835558001
N3254-1G	04061826691939
N3254-5G	04061834115885
N3254-25G	04061833631027