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Merck
CN

N8017

Sigma-Aldrich

Nitrocellulose membranes for blotting

pore size 0.2 μm, size 20 cm × 20 cm

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About This Item

MDL number:
MFCD00081525
UNSPSC Code:
12352200

Key Documents

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size

20 cm × 20 cm

pore size

0.2 μm

SMILES string

OC[C@@H]1O[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](O)O[C@H]2CO)[C@@H](O)[C@H](O)[C@H]1O.[O-][N+](=O)OC[C@@H]3O[C@H](O[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H]3O[C@H]4O[C@@H](CO[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@@H]4O[N+]([O-])=O

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Application

Nitrocellulose membranes are the most popular membranes for immunoblotting (Western blotting) of proteins. The basis of protein binding to nitrocellulose is believed to be due to hydrophobic interactions. The 0.45 μm pore size is the most commonly used for proteins greater than 10,000 MW, but for smaller proteins 0.2 μm is recommended to more efficiently trap the proteins.

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H228

Precautionary Statements

P210

Hazard Classifications

Flam. Sol. 1

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Guillermo Baena et al.
Plants (Basel, Switzerland), 10(1) (2020-12-31)
Phosphoenolpyruvate carboxylase (PEPC) is an enzyme with key roles in carbon and nitrogen metabolisms. The mechanisms that control enzyme stability and turnover are not well known. This paper investigates the degradation of PEPC via selective autophagy, including the role of
Agnieszka Mykowska et al.
Nucleic acids research, 39(20), 8938-8951 (2011-07-29)
Mutant transcripts containing expanded CUG repeats in the untranslated region are a pathogenic factor in myotonic dystrophy type 1 (DM1). The mutant RNA sequesters the muscleblind-like 1 (MBNL1) splicing factor and causes misregulation of the alternative splicing of multiple genes
Sunniva Stordal Bjørklund et al.
BMC cancer, 15, 524-524 (2015-07-18)
Alternate transcripts from a single gene locus greatly enhance the combinatorial flexibility of the human transcriptome. Different patterns of exon usage have been observed when comparing normal tissue to cancers, suggesting that variant transcripts may play a role in the

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