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Merck
CN

N8392

Nitrocellulose membranes for blotting

pore size 0.45 μm, size 20 cm × 20 cm

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About This Item

UNSPSC Code:
12352200
MDL number:
MFCD00081525

Key Documents

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SMILES string

OC[C@@H]1O[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](O)O[C@H]2CO)[C@@H](O)[C@H](O)[C@H]1O.[O-][N+](=O)OC[C@@H]3O[C@H](O[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H]3O[C@H]4O[C@@H](CO[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@@H]4O[N+]([O-])=O

size

20 cm × 20 cm

pore size

0.45 μm

Application

Nitrocellulose membranes are the most popular membranes for immunoblotting (Western blotting) of proteins. The basis of protein binding to nitrocellulose is believed to be due to hydrophobic interactions. The 0.45 μm pore size is the most commonly used for proteins greater than 10,000 MW, but for smaller proteins 0.2 μm is recommended to more efficiently trap the proteins.

pictograms

Flame
GHS02

signalword

Danger

hcodes

H228

pcodes

P210

Hazard Classifications

Flam. Sol. 1

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
122H1074

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Agnieszka Mykowska et al.
Nucleic acids research, 39(20), 8938-8951 (2011-07-29)
Mutant transcripts containing expanded CUG repeats in the untranslated region are a pathogenic factor in myotonic dystrophy type 1 (DM1). The mutant RNA sequesters the muscleblind-like 1 (MBNL1) splicing factor and causes misregulation of the alternative splicing of multiple genes
Sunniva Stordal Bjørklund et al.
BMC cancer, 15, 524-524 (2015-07-18)
Alternate transcripts from a single gene locus greatly enhance the combinatorial flexibility of the human transcriptome. Different patterns of exon usage have been observed when comparing normal tissue to cancers, suggesting that variant transcripts may play a role in the

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