N9411
Nerve growth factor receptor/Fc chimera from mouse
recombinant, expressed in mouse NSO cells, lyophilized powder, suitable for cell culture
Synonym(s):
NGF R, p75 Neurotrophin Receptor
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About This Item
UNSPSC Code:
12352200
MDL number:
recombinant
expressed in mouse NSO cells
assay
≥90% (SDS-PAGE)
form
lyophilized powder
mol wt
apparent mol wt 75-100 kDa by SDS-PAGE (reducing), calculated mol wt 50.4 kDa
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Ngfr(18053)
Biochem/physiol Actions
The NGF receptor also named low-affinity NGF receptor (LNGFR), a potent NGF antagonist, is a 75 kDa, type I transmembrane protein consisting of an extracellular domain, which contains four cysteine-rich domains responsible for ligand binding. The cytoplasmic region of the receptor contains a subtype 2 death domain. Nerve Growth Factor Receptor (NGF R), binds NGF and other neurotrophins including BDNF, NT-3, and NT-4 with similar affinity. NGF-R is necessary for regulating neuronal growth, cell migration, gene expression, differentiation, and cell death during development of the central and peripheral nervous system.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
Preparation Note
Nerve growth factor receptor/Fc chimera mouse is a chimeric protein expressed in a mouse myeloma cell line, NSO. A DNA sequence encoding the signal peptide from human CD33 joined with the extracellular domain of mouse nerve growth factor receptor (NGF R)1 is fused to the Fc region of human IgG1 via a peptide linker. Mature recombinant mouse NGF R /Fc is a disulfide linked homodimeric protein. Based on N-terminal amino acid sequencing, the mature protein starts at Gly 17. The recombinant mouse NGF R/Fc monomer has a calculated molecular mass 50.4 kDa. As a result of glycosylation, recombinant mouse NGF receptor/Fc migrates with an apparent molecular mass of 75-100 kDa in SDS-PAGE.
Analysis Note
The biological activity is measured by its ability to inhibit recombinant mouse β-NGF-induced proliferation of TF-1 cells.
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Wea-Lung Lin et al.
Chemico-biological interactions, 188(3), 607-615 (2010-09-15)
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C Tuffereau et al.
The EMBO journal, 17(24), 7250-7259 (1998-12-19)
A random-primed cDNA expression library constructed from the mRNA of neuroblastoma cells (NG108) was used to clone a specific rabies virus (RV) receptor. A soluble form of the RV glycoprotein (Gs) was utilized as a ligand to detect positive cells.
S X Bamji et al.
The Journal of cell biology, 140(4), 911-923 (1998-03-21)
To determine whether the p75 neurotrophin receptor (p75NTR) plays a role in naturally occurring neuronal death, we examined neonatal sympathetic neurons that express both the TrkA tyrosine kinase receptor and p75NTR. When sympathetic neuron survival is maintained with low quantities
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