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About This Item

MDL number:
MFCD01633927
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4F5, monoclonal

form

buffered aqueous solution

mol wt

antigen 14 kDa

species reactivity

human, hamster, monkey, mouse, rat, chicken, Xenopus

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable using 3.7% formaldehyde-0.2% Triton X-100 or 4% paraformaldehyde-methanol
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using HeLa cells cytosol preparation

isotype

IgG2b

UniProt accession no.

P61970

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NUTF2(10204)
mouse ... Ntf2(100043462)
rat ... Nutf2(291981)

General description

Nuclear transport factor 2 (NTF2 or NUTF2) is a cytosolic soluble protein. The polypeptide chain of NTF2 has α and β sheets and a hydrophobic cavity. NUTF2 is localized on the human chromosome on 16q22.1.

Immunogen

recombinant human NTF2. The epitope lies within the extreme C-terminus of NTF2.

Application

Monoclonal Anti-NTF2 antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

Nuclear transport factor 2 (NTF2 or NUTF2) binds with Ras (Rat Sarcoma)-family GTPase (guanosine triphosphatase) Ran (Ras related nuclear protein) and facilitates the transport of Ran to the nucleus. NTF2 binds to nuclear pore complex (NPC) nucleoporin proteins with FxFG repeats especially p62 also binds preferably to GDP-Ran rather than GTP-Ran to form the complex GDP-Ran and p62 binds at different regions in the hydrophobic cavity of NTF2. The mutation in NTF2 hinders the binding and transport of Ran and formation the p62-NTF2-Ran complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

The antibody may also be used for microinjection, in vitro nuclear protein import assays, and blocking of NTF2 binding to Ran.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
051K4895

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