N9876
4-Nitrophenyl butyrate
lipase substrate, chromogenic, esterase substrate, ≥98%, liquid
Synonym(s):
p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester
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About This Item
Empirical Formula (Hill Notation):
C10H11NO4
CAS Number:
Molecular Weight:
209.20
Beilstein:
1879729
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
Product Name
4-Nitrophenyl butyrate, ≥98%
Quality Level
Assay
≥98%
form
liquid
density
1.19 g/mL at 20 °C (lit.)
storage temp.
−20°C
SMILES string
CCCC(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3
InChI key
DVDUMIQZEUTAGK-UHFFFAOYSA-N
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General description
4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.
Application
4-nitrophenyl butyrate has been used:
- as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene
- as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity
- as a substrate to determine the effect of buffers and solvents on human carboxylesterase
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Monitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate
Pliego J, et al.
Sensors, 15(2), 2798-2811 (2015)
Nathalia S Rios et al.
Biochimica et biophysica acta. Proteins and proteomics, 1867(9), 741-747 (2019-06-16)
The lipase from Pseudomonas fluorescens (PFL) has been immobilized on glyoxyl-octyl agarose and compared to the enzyme immobilized on octyl-agarose. Thus, PFL was immobilized at pH 7 on glyoxyl-octyl support via lipase interfacial activation and later incubated at pH 10.5 for 20 h
Differential response to chemically altered polyethylene by activated mature human monocyte-derived macrophages
Xing S, et al.
Biomaterials, 23(17), 3595-3602 (2002)
Sara Arana-Peña et al.
Frontiers in bioengineering and biotechnology, 8, 36-36 (2020-03-18)
The lipase from Pseudomonas fluorescens (PFL) has been immobilized on octyl-agarose beads under 16 different conditions (varying pH, ionic strength, buffer, adding some additives) at two different loadings, 1 and 60 mg of enzyme/g of support with the objective of
Li-Chun Wang et al.
Journal of food and drug analysis, 26(3), 973-984 (2018-07-07)
Obesity is associated with higher risks of developing diabetes and cardiovascular disease. Green tea, rich in polyphenolic compounds such as epigallocatechin gallate (EGCG) and epigallocatechin (EGC), has been shown to display anti-obesity effects. Houttuynia cordata leaves have also been shown
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