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Merck
CN

N9876

4-Nitrophenyl butyrate

lipase substrate, chromogenic, esterase substrate, ≥98%, liquid

Synonym(s):

p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C10H11NO4
CAS Number:
Molecular Weight:
209.20
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
1879729
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Product Name

4-Nitrophenyl butyrate, ≥98%

Quality Segment

assay

≥98%

form

liquid

density

1.19 g/mL at 20 °C (lit.)

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3

InChI key

DVDUMIQZEUTAGK-UHFFFAOYSA-N

General description

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.

Application

4-nitrophenyl butyrate has been used:
  • as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene
  • as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity
  • as a substrate to determine the effect of buffers and solvents on human carboxylesterase



pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



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