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Merck
CN

O7505

Sigma-Aldrich

Orcein

certified by the Biological Stain Commission, powder

Synonym(s):

Natural Red 28

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About This Item

CAS Number:
1400-62-0
EC Number:
215-750-6
MDL number:
MFCD00062310
UNSPSC Code:
12171500
NACRES:
NA.47

Key Documents

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Product Name

Orcein, synthetic, certified by the BSC

biological source

synthetic

Quality Level

200

Agency

certified by the BSC

form

powder

technique(s)

microbe id | staining: suitable

solubility

1 M NaOH: 1 mg/mL

εmax

≥250% at 574-582 nm in NaOH at 0.01 M

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

N(c5c(cc(cc5C)O)O)C1=C(c2[c]([o][c]3c2C(=C(C(=O)C=3)Nc4c(cc(cc4C)O)O)C)=CC1=O)C

InChI

1S/C28H24N2O7/c1-11-5-15(31)7-17(33)25(11)29-27-13(3)23-21(9-19(27)35)37-22-10-20(36)28(14(4)24(22)23)30-26-12(2)6-16(32)8-18(26)34/h5-10,29-34H,1-4H3

InChI key

VPEASJIRGSVXBF-UHFFFAOYSA-N

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General description

Orcein (Cl Natural Red 28) is a versatile dye known for its exceptional staining properties, particularly for elastic fibres and lamellae in histological samples. With an absorption maximum of 575 nm in an aqueous solution at pH 9.2 and an emission maximum of 585-590 nm, Orcein is widely used in histology and cytology for its ability to highlight connective tissue elements. The dye exhibits varied solubility depending on the batch and pH, ranging from practically insoluble to 2% soluble in water, and from 0.1% to 4% in ethanol.

Application

Certified for use in Darrow′s elastic fiber stain; Orcein method for hepatitis B surface antigen.
Orcein is suitable for various applications, including:
  • Staining Elastic Fibers: Commonly used to stain elastic fibers and lamellae in tissue sections, facilitating the study of connective tissue architecture.
  • Whole Mount and Cryosection Staining: Effective for staining whole mounts and cryosections, allowing for detailed visualization of tissue morphology.
  • Quantification of Elastin: Utilized in quantifying elastin content in tissues, which is vital for understanding various pathological conditions.
  • Fluorescent Staining: Employed for fluorescent staining of elastic fibres in epoxy resin sections, enhancing the detection of specific tissue components.
  • Demonstration of Connective Tissue Elements: Used to visualize related connective tissue structures, including elaunin and oxytalan fibers.
  • Chromosome Staining: Applied in the staining of chromosomes in plants and human sex chromatin, aiding in genetic and cytogenetic studies.
  • Nuclear Staining: Serves as a nuclear stain for whole mount staining of embryos and assessing oocyte maturation, contributing to developmental biology research.
  • In-Situ Hybridization: Recommended for determining stages of meiosis in plant meiocytes, enhancing genetic research capabilities.
  • Hepatic Inclusions Demonstration: Useful in demonstrating hepatic inclusions, particularly hepatitis B antigen and copper-protein deposits, aiding in liver pathology studies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT1465
SHBS3498
SHBR3683
SHBQ8527
SHBP6893

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Kamelija Zarkovic et al.
Redox biology, 4, 109-117 (2015-01-02)
Vascular aging is associated with structural and functional modifications of the arteries, and by an increase in arterial wall thickening in the intima and the media, mainly resulting from structural modifications of the extracellular matrix (ECM) components. Among the factors
Sharma AK and Sharma A
Chromosome Techniques: Theory and Practice (2014)
Elastofibroma dorsi.
Jan Janzen
Diagnostic cytopathology, 35(5), 311-312 (2007-04-12)
Ying-Hua Xuan et al.
The Korean journal of parasitology, 45(1), 1-9 (2007-03-22)
The endosymbionts of 4 strains of Acanthamoeba (KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and 16S rDNA sequence analysis. Double membrane-bound, rod-shaped endosymbionts were distributed
István Sejben et al.
Journal of clinical pathology, 63(7), 575-578 (2010-05-27)
To assess venous invasion (VI) and its relation to distant metastases in colorectal cancer (CRC). Primary untreated CRC cases were assessed for VI. All tumour blocks were stained with H&E and orcein. The presence of VI and nodal status were
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