P0520
Phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart
lyophilized powder (contains imidazole buffer salts and magnesium sulfate), >0.1 units/mg protein (with added activator), PI fraction of Ho, H.C., et al., Biochim. Biophys. Acta, 429, 461 (1976).
Synonym(s):
3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine heart, PDE
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About This Item
Specific activity:
>0.1 units/mg protein (with added activator)
Biological source:
bovine heart
biological source
bovine heart
form
lyophilized powder (contains imidazole buffer salts and magnesium sulfate)
specific activity
>0.1 units/mg protein (with added activator)
mol wt
~60 kDa
composition
Protein, ~75%
UniProt accession no.
foreign activity
5′-ATPase, alkaline phosphatase, and 2′:3′-cyclic nucleotide phosphodiesterase <0.005 units/mg protein, 5′-nucleotidase <0.01 units/mg protein, inorganic pyrophosphatase <0.5 units/mg protein
storage temp.
−20°C
Gene Information
cow ... PDE1A(281969)
Application
Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca2+. It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold.
For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.
Biochem/physiol Actions
Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.
The herbicide Atrazine was shown inhibit phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart.
Packaging
Package size based on activated units
Preparation Note
Crude preparation which has been stripped of the protein activator, calmodulin.
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. If stored at 4 °C, both the activated and activator deficient activity, may decrease approx. 30% in 24 hrs.
Analysis Note
Protein determined by Warburg-Christian unless specified otherwise.
Other Notes
One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Mark Roberge et al.
Toxicology letters, 154(1-2), 61-68 (2004-10-12)
Atrazine (ATR), 2-chloro-4-ethylamino-6-isopropylamino-s-triazine, has been implicated in numerous studies to act as an endocrine disruptor, specifically by altering estradiol signaling via increased aromatase activity. Fluorescence polarization (FP) was used to show that the binding equilibria between estrogen receptor-alpha or estrogen
C Y Huang et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(2), 871-874 (1981-02-01)
Kinetic studies on the activation of cyclic nucleotide phosphodiesterase (3',5'-cyclic-nucleotide 5'-nucleotidohydrolase, EC 3.1.4.17) as a function of calmodulin and Ca2+ concentrations have been carried out. A general approach to analyzing the mechanism of activation, which takes into consideration the various
Binding of trifluoperazine to the calcium-dependent activator of cyclic nucleotide phosphodiesterase.
R M Levin et al.
Molecular pharmacology, 13(4), 690-697 (1977-07-01)
T Kaneko et al.
Cellular signalling, 7(5), 527-534 (1995-07-01)
Elevated intracellular cyclic AMP is associated with the inhibition of many inflammatory cellular responses. In this study, we examined the effect of cyclic AMP on eosinophil chemotaxis. Eosinophils were isolated from healthy human volunteers using an immunomagnetic method. Eosinophils were
Evidence that calmodulin may be involved in phytohaemagglutinin-stimulated lymphocyte division.
S Mac Neil et al.
Bioscience reports, 2(11), 891-897 (1982-11-01)
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