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Merck
CN

P0690

Pectinase from Aspergillus niger

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

Synonym(s):

Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger

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About This Item

CAS Number:
9032-75-1
UNSPSC Code:
12352200
EC Number:
232-885-6
MDL number:
MFCD00131809
EC Number:
3.2.1.15 (BRENDA, IUBMB)

Key Documents

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Product Name

Pectinase from Aspergillus niger, BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

product line

BioReagent

form

aqueous glycerol solution

specific activity

≥5 units/mg protein (Lowry)

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

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Application

Pectinase P0690 is a natural pectinase (polygalacturonase) produced by Aspergillus niger. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Other Notes

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Physical form

Solution in 40% glycerol

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
027K0698
024K07321
024K07322
126H134225

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Vincenzo Lionetti et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(2), 616-621 (2010-01-19)
Plant cell walls represent an abundant, renewable source of biofuel and other useful products. The major bottleneck for the industrial scale-up of their conversion to simple sugars (saccharification), to be subsequently converted by microorganisms into ethanol or other products, is
Sina Pricelius et al.
Journal of agricultural and food chemistry, 57(3), 1006-1012 (2009-02-05)
Attractive color is one of the most important sensory characteristics of fruit and berry products, and elderberry juice is widely used as natural colorant. When pectinase preparations were used in the production of elderberry juice for clarification, a concomitant decrease
Mi Wei et al.
Bioresource technology, 146, 549-555 (2013-08-27)
A novel method utilizing microbial treatment for cleaner production of diosgenin from Dioscorea zingiberensis C.H. Wright (DZW) was presented. A new Bacillus pumilus HR19, which has the great ability to secrete pectinase, was screened and applied in the microbial treatment.
Sara Posé et al.
Journal of experimental botany, 64(12), 3803-3815 (2013-07-23)
Antisense-mediated down-regulation of the fruit-specific polygalacturonase (PG) gene FaPG1 in strawberries (Fragaria×ananassa Duch.) has been previously demonstrated to reduce fruit softening and to extend post-harvest shelf life, despite the low PG activity detected in this fruit. The improved fruit traits
Isabelle Benoit et al.
BMC genomics, 13, 321-321 (2012-07-21)
Pectins are diverse and very complex biomolecules and their structure depends on the plant species and tissue. It was previously shown that derivatives of pectic polymers and oligosaccharides from pectins have positive effects on human health. To obtain specific pectic
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