P1609
Protein Kinase C Catalytic Subunit from rat brain
≥90% (SDS-PAGE), ≥800 units/mg protein
Synonym(s):
PKC-M, PKM
Assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥800 units/mg protein
packaging
vial of 200 ng
concentration
25 μg/mL protein
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
rat ... Prkd1(85421)
Biochem/physiol Actions
Does not require Ca2+ or phosphatidylserine for its activity.
Physical form
Storage buffer: 20 mM Tris-HCl, pH 7.5, 2 mM EGTA, 2 mM EDTA, 1 mM DTT, 10 mM potassium phosphate, 50% glycerol, 0.05% Triton™ X-100
Preparation Note
Prepared by tryptic digestion of PKC.
Other Notes
One unit will transfer of 1 nanomole of phosphate per minute at 30 °C using Histone III as substrate and phosphatidylserine as an activator.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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B VanRenterghem et al.
The Journal of biological chemistry, 269(40), 24666-24672 (1994-10-07)
Previously pp60v-src, cyclin A, p39mos, and maturation-promoting factor (composed of Cdc2 and cyclin B) have been shown to activate mitogen-activated protein kinase (MAPK) and MAPK kinase (MEK) in cell-free extracts of Xenopus oocytes. The pp60v-src pathway is dependent on a
K P Huang et al.
Biochemical and biophysical research communications, 139(1), 320-326 (1986-08-29)
Tryptic fragments of protein kinase C containing the kinase (45 KDa) and phorbol ester-binding activity (38 KDa) were separated by Mono O column chromatography. The purified phorbol ester-binding fragment exhibits a higher affinity for phosphatidylserine than the native enzyme but
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