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P1790

Phosphodiesterase, 3′,5′-Cyclic Nucleotide, Activator-deficient from porcine brain

lyophilized powder

Synonym(s):

3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from porcine brain, PDE

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About This Item

UNSPSC Code:
12352200
MDL number:
MFCD00131864
EC Number:
3.1.4.17 (BRENDA, IUBMB)
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form

lyophilized powder

specific activity

1.0-5.0 units/mg protein (without added calmodulin.)

mol wt

~60 kDa

purified by

affinity chromatography

contains

lactose as stabilizer

composition

Protein, ~5% Lowry

UniProt accession no.

Q9XSW7

foreign activity

5′-ATPase, inorganic pyrophosphatase, alkaline phosphatase and 2′,3′-cyclic nucleotide phosphodiesterase <0.1%, 5′-nucleotidase <0.5%

storage temp.

−20°C

Gene Information

pig ... PDE3A(396555)

Application

May be used to assay the protein activator, calmodulin.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Packaging

Package size based on activated units.

Physical form

Lyophilized preparation which has been depleted of calmodulin and containing buffer salts as Tris-HCl.

Preparation Note

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.

Analysis Note

Protein determined by Warburg-Christian unless specified otherwise.

Other Notes

One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
028F9670
028F9665

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Chimed Jansen et al.
Journal of medicinal chemistry, 56(5), 2087-2096 (2013-02-16)
Trypanosoma brucei cyclic nucleotide phosphodiesterase B1 (TbrPDEB1) and TbrPDEB2 have recently been validated as new therapeutic targets for human African trypanosomiasis by both genetic and pharmacological means. In this study we report the crystal structure of the catalytic domain of
N B Matveeva et al.
Biofizika, 57(5), 832-839 (2012-11-10)
Possible involvement of autocrine factors into the control of motile behavior via a receptor-mediated mechanism was investigated in Physarum polycephalum plasmodium, a multinuclear amoeboid cell with the auto-oscillatory mode of motility. Cyclic adenosine monophosphate (cAMP) and extracellular cAMP-specific phosphodiesterase, its
Tamam El-Elimat et al.
Journal of natural products, 76(3), 382-387 (2013-01-11)
Three bioactive compounds were isolated from an organic extract of an ascomycete fungus of the order Chaetothyriales (MSX 47445) using bioactivity-directed fractionation as part of a search for anticancer leads from filamentous fungi. Of these, two were benzoquinones [betulinan A
Cheng Fang et al.
Leukemia research, 37(5), 536-540 (2013-03-05)
In order to determine the relationship between expression of cyclic nucleotide phosphodiesterase isoform 7B (PDE7B) and mantle cell lymphoma (MCL) progression, PDE7B mRNA expression was measured by qRT-PCR in 21 untreated MCL patients with bone marrow involvement and 20 healthy
Sanja Beca et al.
Circulation research, 112(2), 289-297 (2012-11-22)
cAMP is an important regulator of myocardial function, and regulation of cAMP hydrolysis by cyclic nucleotide phosphodiesterases (PDEs) is a critical determinant of the amplitude, duration, and compartmentation of cAMP-mediated signaling. The role of different PDE isozymes, particularly PDE3A vs
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