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Merck
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P1790

Sigma-Aldrich

Phosphodiesterase, 3′,5′-Cyclic Nucleotide, Activator-deficient from porcine brain

lyophilized powder

Synonym(s):

3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from porcine brain, PDE

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About This Item

EC Number:
3.1.4.17 (BRENDA, IUBMB)
MDL number:
MFCD00131864
UNSPSC Code:
12352200

Key Documents

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form

lyophilized powder

specific activity

1.0-5.0 units/mg protein (without added calmodulin.)

mol wt

~60 kDa

purified by

affinity chromatography

contains

lactose as stabilizer

composition

Protein, ~5% Lowry

UniProt accession no.

Q9XSW7

foreign activity

5′-ATPase, inorganic pyrophosphatase, alkaline phosphatase and 2′,3′-cyclic nucleotide phosphodiesterase <0.1%
5′-nucleotidase <0.5%

storage temp.

−20°C

Gene Information

pig ... PDE3A(396555)

Application

May be used to assay the protein activator, calmodulin.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Packaging

Package size based on activated units.

Physical form

Lyophilized preparation which has been depleted of calmodulin and containing buffer salts as Tris-HCl.

Preparation Note

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.

Analysis Note

Protein determined by Warburg-Christian unless specified otherwise.

Other Notes

One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
028F9670
028F9665

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The cyclic AMP pathway.
Paolo Sassone-Corsi
Cold Spring Harbor perspectives in biology, 4(12), doi:10-doi:10 (2012-12-05)
Ryan T Cameron et al.
Biochemical pharmacology, 85(9), 1297-1305 (2013-03-12)
PDE4 is one of eleven known cyclic nucleotide phosphodiesterase families and plays a pivotal role in mediating hydrolytic degradation of the important cyclic nucleotide second messenger, cyclic 3'5' adenosine monophosphate (cAMP). PDE4 inhibitors are known to have anti-inflammatory properties, but
Koji Ochiai et al.
Bioorganic & medicinal chemistry letters, 23(1), 375-381 (2012-12-04)
(-)-6-(7-Methoxy-2-(trifluoromethyl)pyrazolo[1,5-a]pyridin-4-yl)-5-methyl-4,5-dihydropyridazin-3(2H)-one (KCA-1490) exhibits moderate dual PDE3/4-inhibitory activity and promises as a combined bronchodilatory/anti-inflammatory agent. N-alkylation of the pyridazinone ring markedly enhances potency against PDE4 but suppresses PDE3 inhibition. Addition of a 6-aryl-4,5-dihydropyridazin-3(2H)-one extension to the N-alkyl group facilitates both enhancement
Stephen M Kraynik et al.
Molecular pharmacology, 83(6), 1155-1165 (2013-03-16)
Brown adipose tissue (BAT) is a highly thermogenic organ that converts lipids and glucose into heat. Many of the metabolic and gene transcriptional hallmarks of BAT activation, namely increased lipolysis, uncoupling protein-1 (UCP1) mRNA, and glucose uptake, are regulated by
One single signaling pathway for so many different biological functions: lessons from the cyclic adenosine monophosphate/protein kinase A pathway-related diseases.
Guillaume Assié
The Journal of clinical endocrinology and metabolism, 97(12), 4355-4357 (2012-12-12)
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