P2065
Purothionin from whole wheat flour
Mixture of α- and β-isomers, lyophilized powder, 0.25-1.0 units/mg protein
biological source
whole wheat flour
form
lyophilized powder
specific activity
0.25-1.0 units/mg protein
composition
Protein, ~90%
UniProt accession no.
storage temp.
−20°C
Gene Information
wheat ... Pur-B1(543304)
Biochem/physiol Actions
In conjunction with thioredoxin, affects proper protein folding; cytotoxic, presumably by forming pores in cytoplasmic membrane.
Other Notes
One unit will cause a ΔA650 of 1.0 per min (increase the turbidity) of a 1 mg/ml solution of bovine insulin in the presence of 2 mM EDTA and 0.33 mM DTT at pH 7.5 at 37 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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M F De Bolle et al.
Electrophoresis, 12(6), 442-444 (1991-06-01)
A technique was developed for the detection of antifungal activity of proteins after discontinuous polyacrylamide gel electrophoresis under native conditions. The antifungal activity is detected as growth inhibition zones in a homogeneous fungal lawn, grown in an agar layer spread
Purification and partial characterization of a 31-kDa cysteine endopeptidase from germinated barley.
N Zhang et al.
Planta, 199(4), 565-572 (1996-01-01)
Proteolytic enzymes hydrolyze cereal seed storage proteins into small peptides and amino acids, which are very important for seed germination and the malting process. A cysteine-class endopeptidase was purified from 4-d-germinated barley (Hordeum vulgare L. cv. Morex). Four purification steps
Okumura, K., et al.
Agricultural and Biological Chemistry, 53, 3303-3303 (1989)
M Bruix et al.
Biochemistry, 32(2), 715-724 (1993-01-19)
The complete assignment of the proton NMR spectra of the homologous gamma 1-hordothionin and gamma 1-purothionin (47 amino acids, 4 disulfide bridges) from barley and wheat, respectively, has been performed by two-dimensional sequence-specific methods. A total of 299 proton-proton distance
T C Johnson et al.
Plant physiology, 85(2), 446-451 (1987-10-01)
Thioredoxin h, the thioredoxin characteristic of heterotrophic plant tissues, was purified to homogeneity from wheat endosperm (flour) and found to resemble its counterpart from carrot cell cultures. In the presence of NADPH, homogeneous thioredoxin h and partially purified wheat endosperm
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