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Merck
CN

P2621

Phosphomannose Isomerase from Escherichia coli

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein

Synonym(s):

D-Mannose-6-phosphate ketol-isomerase, Mannose Phosphate Isomerase, PMI

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
5.3.1.8 (BRENDA, IUBMB)
MDL number:
MFCD00131899
Specific activity:
≥50 units/mg protein
Recombinant:
expressed in E. coli

Key Documents

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Product Name

Phosphomannose Isomerase from Escherichia coli, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein

recombinant

expressed in E. coli

form

ammonium sulfate suspension

specific activity

≥50 units/mg protein

storage temp.

2-8°C

Quality Level

200

Application

PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).

Biochem/physiol Actions

Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a link between glucose metabolism and mannosylation.

Other Notes

One unit will convert 1.0 μmole of D-mannose 6-phosphate to D-fructose 6-phosphate per min at pH 7.6 at 25 °C, using a coupled enzyme system with phosphoglucose isomerase and glucose-6-phosphate dehydrogenase.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Physical form

Supplied as a suspension in 3.2 M ammonium sulfate

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN6338
BCCM7045
BCCM6485
BCCG7460
BCCC1186

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Shanna Sichwart et al.
Applied and environmental microbiology, 77(4), 1325-1334 (2010-12-21)
The gram-negative facultative chemolithoautotrophic bacterium Ralstonia eutropha strain H16 is known for its narrow carbohydrate utilization range, which limits its use for biotechnological production of polyhydroxyalkanoates and possibly other products from renewable resources. To broaden its substrate utilization range, which
Craig T Narasaki et al.
PloS one, 6(10), e25514-e25514 (2011-11-09)
Coxiella burnetii, the etiologic agent of human Q fever, is a gram-negative and naturally obligate intracellular bacterium. The O-specific polysaccharide chain (O-PS) of the lipopolysaccharide (LPS) of C. burnetii is considered a heteropolymer of the two unusual sugars β-D-virenose and
Russell Dahl et al.
Journal of medicinal chemistry, 54(10), 3661-3668 (2011-05-05)
We report the discovery and validation of a series of benzoisothiazolones as potent inhibitors of phosphomannose isomerase (PMI), an enzyme that converts mannose-6-phosphate (Man-6-P) into fructose-6-phosphate (Fru-6-P) and, more importantly, competes with phosphomannomutase 2 (PMM2) for Man-6-P, diverting this substrate
Michael Newcomb et al.
Applied microbiology and biotechnology, 90(2), 625-634 (2011-02-15)
Clostridium thermocellum, an anaerobic, thermophilic, and ethanogenic bacterium produces a large cellulase complex termed the cellulosome and many free glycosyl hydrolases. Most cellulase genes scatter around the genome. We mapped the transcripts of the six-gene cluster celC-glyR3-licA-orf4-manB-celT and determined their
Studies on the phosphomannose isomerase of Amorphophallus konjac C. Koch II. Effect of divalent metal ions on the EDTA-treated enzyme
Takao Murata
Plant & Cell Physiology, 16, 963-970 (1975)
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